Test on apples

Apples with uniform degrees of ripeness and size are used for the tests. Before testing, the apples are surface-sterilized. Four equally-spaced wounds are made per apple. Two wounds are inoculated with the fungus, into the other two wounds the suspension of fungus and the testing preparation is pipetted. The incubation time depends on the storage temperature and the growth rate of the pathogens in the control wounds.

The evaluation is carried out by determining the diameters of the rotten spots. The percentage reduction of the rotten spots compared to the pathogen control is calculated for each sample. This principle can be used to determine whether a preparation has an effect on decay fungi.

Germination test

For Monilia spp. the efficacy of preparations on germination can be determined by a fast in vitro germination test. For this conidia of infectios apples are resuspenden und place on a microscope slide. Preparations are added in appropriate concentration. The slides are incubated for 4 hours at 20°C  under humid conditions. The evaluation is done quantitaively by counting germinated und ungerminated conidia.